Petite H(1), Frei V, Huc A, Herbage D. Author information: (1)Coletica, Lyon, France. Cross-linking of collagen-based biomaterials increases their strength and. By DANIEL HERBAGE, JOSETTE BOUILLET and JEAN-CLAUDE Solubilization of collagen from bovine articular cartilage with pepsin. The formation of new bonds between the denatured collagen chains and the proteoglycans can explain the D. Herbage, A. Huc, D. Chabrand, M.C. Chapuy.

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With the rapid development in tissue engineering, these different biomaterials have been used as three-dimensional scaffolds and cell transplant devices. Immunologically active biomaterials for cancer therapy.

Use of diphenylphosphorylazide for cross-linking collagen-based biomaterials.

Topics Discussed in This Paper. Have you forgotten your login? In this report, we propose a simpler, faster way to prepare acyl azide groups and to cross-link collagen-based biomaterials, using diphenylphosphorylazide DPPA collage a reagent. Daniel Herbage 1 AuthorId: Showing of 84 references.

HerbzgeDr D. Cross-linking of dermal sheep collagen using a water-soluble carbodiimide. Allogeneic Mesenchymal Stem Cells and Biomaterials: Mineral deposition Glycosaminoglycan deposition Articular cartilage repair Collagen-based biomaterial Collagen synthesis.

Collagen References

BiomaterialsElsevier,22, pp. Collagen-based biomaterials in the form of sponges bovine type I collagen, both native and cross-linked by treatment with diphenylphosphorylazide, noted control and DPPA sponges respectively were tested as three-dimensional scaffolds to support chondrocyte proliferation with maintenance of the phenotype in order to form neocartilage. Showing of 71 extracted citations.

Similar decreases in free primary amino group content and increases in thermal stability were obtained for collagen films treated with DPPA, glutaraldehyde, or hydrazine. Collagen-based biomaterials as 3D scaffold for cell cultures: RosenthalGabriele J Koehler Anticancer research Vacanti Journal of cellular biochemistry. Glycosaminoglycans accumulated in the matrix, up to 1. Ham A Benghuzzi Biomedical sciences instrumentation Files produced by the author s. Both supports were always present during the study and a partial decrease in size and weight was detected only with control sponges, both seeded and unseeded.


After determining the optimal conditions of cross-linking with DPPA, we compared the efficiency of this protocol with that using hydrazine and with the classical glutaraldehyde treatment.

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These in vitro results indicate that both collagen matrices can support the development of tissue engineered cartilage. Citations Publications citing this paper. Specific cartilage collagens types II and XI were deposited in the matrix throughout the culture and traces of type I collagen were noticed only in the culture medium after weeks and 4 weeks in the case of 10 6 and 10 7 hegbage sponges, respectively.

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Use of diphenylphosphorylazide for cross-linking collagen-based biomaterials.

Native and DPPA cross-linked collagen sponges seeded with fetal bovine epiphyseal chondrocytes used for cartilage tissue engineering. Monday, October 29, – 9: AliDavid J Mooney Current topics in microbiology and immunology The Perfect Match for Cardiac Repair? DPPA-treated pericardium had a hrebage to collagenase digestion similar to that of glutaraldehyde- or hydrazine-treated pericardium. In order to validate and quantitate the extent of reaction, the degree herbagw cross-linking was determined by the measure of the free primary amino group content of the samples.


These results were corroborated by resistance to bacterial collagenase digestion: References Publications referenced by this paper. By clicking accept or continuing to use the site, you agree to the terms outlined in our Privacy PolicyTerms of Serviceand Dataset License. Metals Organ Ceramics Chitosan polylactic acid-polyglycolic acid copolymer. Development and characterisation of a three-dimensional in vitro hepatic fish model to investigate xenobiotic herbbage Matthew G Baron Application of Collagen Scaffold in Tissue Engineering: Cytological evaluation of capsular tissue surrounding TCPL implant in adult rats.

Chevallay and Dr D. Recently, we described an efficient cross-linking process via colalgen formation of acyl azide groups on methylated carboxyl groups of collagen using hydrazine and nitrous acid referred to here as the hydrazine method. Cross-linking of collagen-based biomaterials increases their strength and hefbage in vivo.

Methods for the treatment of collagenous tissues for bioprostheses. Wednesday, December 13, – 2: Howard The Journal of biological chemistry Aluminum Oxide Search for additional papers on this topic.

Treatment of native collaen pericardium with 0. Cell proliferation was only noted in the 10 6 cells-seeded sponges 4-fold increase after 4 weeks of culture. Mouse fibroblasts in long-term culture within collagen three-dimensional scaffolds: Gelatine-Based Biomaterials and Their Biocompatibility: Gelatinase A activation is regulated by the organization of the polymerized actin cytoskeleton.

Anne-Marie Freyria 1 AuthorId: Collagen as matrix for neo-organ formation by gene-transfected fibroblasts.